Activation and Monitoring of mtDNA Damage in Cancer Cells via the “Proton-Triggered” Decomposition of an Ultrathin Nanosheet

Mitochondrial DNA (mtDNA) damage is a very important molecular event, which has significant effects on living organisms. Therefore, particularly challenge for biomaterials research to develop functionalized nanoparticles that can activate and monitor mtDNA instigate cancer cell apoptosis, as such eliminate the negative Toward goal, with this research, we have developed hydroxyapatite ultrathin nanosheet (HAP-PDCns)—a high Ca2+ content biomaterial. HAP-PDCns undergoes proton-triggered decomposition after entering cells via clathrin-mediated endocytosis, then, it selectively concentrates in charged mitochondrial membrane. This kind of phenomenon facilitates by causing instantaneous local calcium overload mitochondria cells, inhibits tumor growth. Importantly, at same time, real-time green-red-green fluorescence change occurs correlates degree deterioration because changes highest occupied orbital–lowest unoccupied orbital energy gaps during process. Significantly, cannot be triggered normal cells. Thus, induce apoptosis death facilitating damage, but does not affect In addition, simultaneously damage. We anticipate design strategy generalized other used positive organisms while eliminating any effects.